You should see the spore water running down. Remove the tinfoil and inoculate the culture medium with the spore syringe through the 4 holes in the lid against the glass of the jar. Let the needle completely cool down during 20-30 seconds.ДЄfter cooling down shake the syringe very well, so the spores will get divided in the syringe again. Sterilize it by heating it with a gas burner until it starts to glow red. A substrate that is too warm can kill the mushroom spores and all preparations will be for nothing.Д«efore you inoculate your rice-flour cakes, you have to sterilize the needle of your syringe first. Cooling the sterilized jars takes at least a couple of hours. Take the jars out of the pan after they have completely cooled down.

Sterilize the jars at 15 psi for 30 minutes. Now it is time to sterilize the substrate. This reduces the chance of contamination.Д¬lose the jars with the lids and cover them with a large double layer of tin foil. Wipe the top clean with a tissue and fill the last centimeter with only vermiculite. Do this very loose the mycelium needs space to develop itself. Fill the jars one by one with the substrate. Mix the dry components vermiculite and the brown rice flour very well. The amounts for substrate ingredients are (by volume): jars with lids with 4 holes in it at the sides.For this method you will need the following:

Instead of brown rice flour you can also use rye flower.

The substrate to use is a mixture of brown rice flour, vermiculite, and water. This method is also called the PF TEK, named after the person who invented it (Psilocybe Fanaticus). According to many, the easiest method to cultivate the Psilocybe cubensis is the riceflourcake method.

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